Fig. 5
Subretinal injection of AD-derived homogenates accelerates the presence of microglia and astrocytes in the retina of TAU58 mice. a, b; Significant differences were observed in the percentage of the percentage of GFAP and Iba1-positive areas in the central retina (One-way ANOVA with Dunnett’s test: GFAP: p = 0,0143, Iba1: 0,0275). c, d: No significant differences were found in the percentage of GFAP and Iba1-positive areas in the peripheral retina (One-way ANOVA with Dunnett’s test: p > 0,05). Representative merged fluorescence images show astrocytes and microglia in the GCL of the central (e-g) and peripheral (h-j) retinas of 6-month-old female TAU58 mice for AD, non-AD/PART, and PBS-injected groups. The merged images display astrocytes (GFAP) in green, microglia (Iba1) in magenta, pTau (pTauS202/T205) in red, and nuclei (Hoechst) in blue. Single-channel images for the merged images in e-j are shown in Fig. S9; a-d’. Arrowheads indicate pTauS202/T205 retinal cytoplasmic inclusions (g, j). Error bars: Min to Max in a, b, c, d. (PBS; n = 10, non-AD/PART; n = 10, AD; n = 10 cases)