Fig. 4

Subretinal injection of the patient-derived homogenates induces pTau seeding in the retina in TAU58 mice. a: Graphical representation of the experiment: Two-month-old female TAU58 mice were injected with PBS, non-AD/PART, and AD human brain homogenates. After 4 months, mice were sacrificed, and immunohistochemistry was conducted to check for the presence of retinal pTau pathology, as well as pTau-positive neurons in the brain b: Significant differences were noted in the percentage of retinal pTau pathology based on the type of seed injected in the right eye, with significant differences observed between PBS vs. non-AD/PART seeds (One-way ANOVA with Dunnett’s test: p = 0.0128), PBS vs. AD seeds (One-way ANOVA with Dunnett’s test: p < 0.0001). c: No significant differences were observed in the percentage of retinal pTau pathology in the non-injected (left) eye. d: In the optic nerve of the injected (right) eye, a multinomial logistic regression model demonstrated an association between the presence of pTau threads (expressed as a percentage of positive cases) and AD brain lysates (p = 0,033; OR = 9,333 [1,193 to 72,991]) e: In the optic nerve of the non-injected (left) eye, a multinomial logistic regression model demonstrated no association between the presence of pTau threads (expressed as a percentage of positive cases) and different types of injected brain lysates. f: No significant differences were found in the number of positive RBPMS cells in the peripheral injected (right) eye. g: Similarly, no significant differences were found in the number of positive RBPMS cells in the peripheral non-injected (left) eye. h: No significant differences were found in the number of positive RBPMS cells in the central injected (right) eye. i: Likewise, no significant differences were found in the number of positive RBPMS cells in the central non-injected (left) eye. Error bars: Min to Max in b, c, f, g, h, i, SEM in d, e (PBS; n = 10, non-AD/PART; n = 10, AD; n = 10 cases)