Fig. 11

Presence of microglia and astrocytes in the V1 of 6-month-old TAU58 mice brain after subretinal injection of brain lysates. a: Significant differences were observed in the percentage of the percentage of GFAP -positive area in the contralateral V1 between PBS- and AD-brain lysate-injected mice, while no significant difference was found between PBS- and non-AD/PART-injected mice (One-way ANOVA with Dunnett’s test, PBS vs AD: p = 0,0130, PBS vs non-AD/PART: p = 0,4174), b: Significant differences were also observed in the percentage of the percentage of Iba1-positive area in the contralateral V1 between PBS- and AD-brain lysate-injected mice, with no significant difference between PBS- and non-AD/PART-injected mice (One-way ANOVA with Dunnett’s test, PBS vs AD: p = 0,0035, PBS vs non-AD/PART: p = 0,6704). Representative merged fluorescence images of the contralateral V1 (c-e) and their higher magnifications (f-h) show astrocytes (GFAP) in green, microglia (Iba1) in magenta, pTau (pTau^S202/T205) in red, and nuclei (Hoechst) in blue. Single-channel images for the merged images in c-h are shown in Fig. S12a-d’. Magnifications of panels f, g, and h are derived from panels c, d, and e, respectively. Error bars: Min to Max in a, b. Scale bar in c applies to panels c-e, and scale bar in f applies to panels f-h. (PBS; n = 10, non-AD/PART; n = 10, AD; n = 10 cases)