Table 1 Antibodies and probes
From: Limbic system synaptic dysfunctions associated with prion disease onset
Antibodies | From | Catalog # | Use (Dilutions) | Host species | Secondary antibodies for WB (Dilutions) | Secondary antibodies for IF (Dilutions) |
|---|---|---|---|---|---|---|
6D11 | BioLegend | 808,001 | WB (1:5 K) | Mouse (Ms) | Goat (Gt) anti Ms HRP (1:5 K) | – |
MAP2 | SynapticSystems | 188,006 | IF(1:500) | Chicken (Ch) | – | Gt anti Ch Alexa Fluor 647 (1:500) |
Synaptophysin | ABCAM | ab8049 | WB (1:5Â K)/IF(1:200) | Mouse | Gt anti Ms HRP (1:5Â K) | Gt anti Ms Alexa Fluor 488 (1:500) |
PSD95 | ABCAM | ab18258 | WB(1:5Â K)/IF(1:200) | Rabbit (Rb) | Gt anti Rb HRP (1:5Â K) | Gt anti Rb Alexa Fluor 488 (1:500) |
Phalloidin | Invitrogen | A12379 | IF(1:500) | – | – | – |
NF-L | Invitrogen | 13–0400 | WB(1:5 K)/IF(1:200) | Mouse | Gt anti Ms HRP (1:5 K) | Gt anti Ms Alexa Fluor 568 (1:500) |
Beta tubulin | ABCAM | ab6046 | IF(1:200) | Rabbit | – | Gt anti Ms Alexa Fluor 647 (1:500) |
Synaptojanin 1 | ABCAM | ab308136 | IF(1:100)/WB(1:1Â K) | Rabbit | Gt anti Rb HRP (1:5Â K) | Gt anti Rb Alexa Fluor 488 (1:500) |
NeuN | ABCAM | ab177487 | WB(1:2 K) | Rabbit | Gt anti Rb HRP (1:5 K) | – |