Fig. 6
From: The X-linked intellectual disability gene CUL4B is critical for memory and synaptic function
CUL4B-deficient mice exhibit morphological and functional abnormalities in synapses. (a) Representative TEM images of the synaptic structure in the CA1 region of the hippocampus in control and CKO mice. (b-e) Quantification of the length of the active zones (b), the curvature of the synaptic interface (c), the thickness of the postsynaptic density (PSD) (d) and the width of the synaptic cleft (e) (N = 36 synapses from 4 CON mice and 42 synapses from 4 CKO mice). (f) Quantification of the width of the synaptic cleft in asymmetric synapses (N = 24 synapses from 4 CON mice and 27 synapses from 4 CKO mice). (g) Quantification of the width of the synaptic cleft in symmetric synapses (N = 12 synapses from 4 CON mice and 15 synapses from 4 CKO mice). (h) Quantification of the number of synaptic vesicles per synaptosome (N = 36 synapses from 4 CON mice and 42 synapses from 4 CKO mice). (i) Representative traces showing evoked AMPAR EPSCs in the CA1 pyramidal neurons of control and CKO mice. (j) Analysis of the input–output (I–O) relationship between AMPAR EPSCs and incremental stimulation intensities (in µA) (N = 12 cells from 4 mice). (k) Input/output slope of each cell (N = 12 cells from 4 mice). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns, not significant