Fig. 5
From: The X-linked intellectual disability gene CUL4B is critical for memory and synaptic function
CUL4B-deficient mice exhibit synapse loss. (a) Golgi-Cox staining for the measurement of synapse density in pyramidal neurons in the hippocampus. Scale bar, 1 μm. (b) Spine density on basal dendrites per 10 μm (N = 50 dendrites from 3 CON mice and 45 dendrites from 3 CKO mice). (c) Percentages of different subtypes, mushroom (M), stubby (S), thin (T) and filiform pseudopodia (F) spines in CON and CKO mice. (N = 50 dendrites from 3 CON mice and 45 dendrites from 3 CKO mice). (d) Synapsin1 and PSD95 immunostaining of DIV14 neurons from CKO and control mice. Scale bars: 100 μm (upper) or 20 μm (lower). (e-g) Statistical analysis of Synapsin1+ (e), PSD95+ (f), and PSD95+/Synapsin1+ synapse density (g) (N = 29 cells from 3 mice). (h) GAD67 and Gephyrin immunostaining of DIV14 neurons from CKO and littermate control mice. Scale bars: 100 μm (upper) or 20 μm (lower). (i-k) Statistical analysis of GAD67+ (i), Gephyrin+ (j), and GAD67+/Gephyrin+ synapse density (k) (N = 12 cells from 3 mice). * P < 0.05; *** P < 0.001; **** P < 0.0001; ns: not significant