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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: An exceptionally rare case of a diffuse midline glioma with concomitant H3.1 K27M and G34R mutations in the HIST1H3C (H3C3) gene

Fig. 2

In vitro patient-derived modelling. (A) Photo of ICR-CXJ074 2D culture, grown on laminin, passage 5 (top). Photo of ICR-CXJ074 3D neurosphere culture, passage 5 (below). (B) Immunofluorescence panel exploring the profiles of ICR-CXJ074 (3D). GFAP (red), Nestin (green), OLIG2 (green), SOX2 (red). (C) Heatmap showing screen results from the ICR drugs & tools library, compared with other screens using histone-mutant and wild-type models. ICR-CXJ74 results are shown in the heatmap on the left. (D) Heatmaps showing profiles of the H3K27me3 (left) and H3K27ac (right) peaks for ICR-CXJ074 compared to other histone-mutant tumours (the different histone subtypes are highlighted by a coloured outline according to the key provided). (E) Oncoprints showing ChIP-Seq gene level comparisons for H3K27ac (left) and H3K27me3 (right) compared to other histone-mutant models

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Acta Neuropathologica Communications

ISSN: 2051-5960

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