Fig. 4

Immunohistochemical analysis shows the immune infiltration characteristics. Comparison of infiltrating density of CD8+ T cells (A, B), CD68+ macrophages (C, D), and CD163+ M2 macrophages (E, F) between high- and low-GYMS group. All tumor slides were stained with antibodies against CD8 (Cat. ab209775, 1:2000, Abcam), CD68(ZM-0060 Ready-to-Use, ZSGB-BIO), and CD163(ZA-0428 Ready-to-Use, ZSGB-BIO). Scale bars: 100 μm in 200x and 50 μm in 400x