Fig. 4
From: Retinal cytoarchitecture is preserved in an organotypic perfused human and porcine eye model
Retinal cytoarchitecture is preserved in perfused human eyes. A-H) A representative panel of human retina sections following 24 h perfusion (Perfused) in comparison to fresh frozen controls (Control). Preserved staining and morphology were observed for (A, E) RBPMS, (B, F) GS, (C, G) Chx-10, (D, H) R/G-Opsin (n = 3). Fresh control and perfused human retinas were counted for I) RGCs (RBPMS), J) bipolar cells (Chx-10), and K) cones (R/G-Opsin) with no significant differences observed (n = 3). (Scale bars indicate 50 μm). L-Q) Retinal tissue was probed for markers of active cellular metabolism (ATPase; L, O, Glut1; M, P) and glial reactivity (GFAP; N, Q). Similar antibody staining profiles were observed between fresh frozen (Control) and perfused human eyes (Perfused). (Scale bar = 50 μm)