Fig. 3
From: Retinal cytoarchitecture is preserved in an organotypic perfused human and porcine eye model
Retinal cell death is reduced by physiological ocular perfusion. A-F) Representative TUNEL (green) and DAPI (blue) and staining of central retinal sections after 24 h perfusion. There was no signal in fresh control retinas, some staining in perfused retinas after 24 h (arrows), but much more positive staining appeared in no-perfusion retinas (scale bars = 50 μm. Note; lower magnification was necessary to accommodate the highly disrupted no-perfusion images, as indicated by the scale bars). E) Quantification of the TUNEL signal from multiple eyes shows significantly less cell death in perfused eyes in each retinal layer when compared to no-perfusion eyes (n = 6). (*p < 0.05, **p < 0.01, ***p < 0.005, bars are S.E)