Fig. 2
From: Retinal cytoarchitecture is preserved in an organotypic perfused human and porcine eye model
Retinal cytoarchitecture and cell survival are preserved in perfused eyes. Retinas from fresh porcine eyes (Control), 24 h anterior perfused eyes (Perfused), and 24 h no-perfusion control (No Perfusion) were sectioned and stained with antibodies directed to a variety of retinal cell markers, which were assessed for cytoarchitectural features. A,E,I) Representative images of RBPMS and Calbindin, B,F,J) Chx-10 and AP-2α, C,G,K) R/G-Opsin and β3-Tub, and D,H,L) GS. The corresponding markers and cytoarchitecture were preserved in retinas following 24 h of anterior perfusion compared to severely disrupted staining in eyes maintained under the same conditions, but without active perfusion (No-Perfusion). (Scale bars = 50 μm, GCL; ganglion cell layer, INL; inner nuclear layer, ONL; outer nuclear layer). M) Counts of RGCs (RBPMS staining) in both central and peripheral retina are not significantly different in perfused eyes compared to fresh controls, in contrast to significantly reduced numbers in non-perfused eyes (n = 6). N) Amacrine cells (AP-2α staining) and (O) bipolar cells (Chx-10 staining) similarly show significant preservation in perfused eyes compared to non-perfused (n = 6). In the central retina there was a significant loss of bipolar cells in perfused compared to fresh control eyes. (*p < 0.05, **p < 0.01, ***p < 0.005), bars are S.E)