Fig. 2
From: Pediatric high-grade gliomas with concomitant RB1 and SETD2 alterations and Li-Fraumeni syndrome
Histopathological and molecular features of cases from this cohort. a-d Both cases presented an astrocytic differentiation with pleomorphic giant cells, necrosis, microvascular proliferation and numerous mitotic figures (HPS, magnification x400). e-f Both showed a loss of RB1 protein expression (magnification x400) and case #2 presented an overexpression of p53 (g, magnification x400). h The FISH analysis evidenced an amplification of both MYCN (orange signals) without ID2 (blue signals) loci amplification (magnification x800) for case #1. Case #1 did not have Li-Fraumeni syndrome. Copy number variations for cases #1 (i) and #2 (j). FISH: Fluorescence in situ hybridization; HPS: Hematoxylin Phloxin Saffron; mut.: mutation. Black scale bars represent 50 μm