Fig. 3

Axonal and dendritic loss of RGCs visualized by NEFL immunolabelling in the peripheral retina and ONH. A Decreased NEFL expression in temporal peripheral retina and ONH, and disorganization of NEFL immune staining was observed in glaucomatous tissues. Asterisks indicate representative areas with loss of NEFL. B Scatter dot plot represents the individual values of neurofilament light chain (NEFL) for non-glaucomatous (retina, n = 16, ONH, n = 16) and glaucomatous donor tissues (retina, n = 21; ONH, n = 18). Quantitation of NEFL staining showed a statistically significant reduction in the temporal peripheral retina (40%) and optic nerve head (42.4%) of glaucoma donors. Horizontal bars indicate mean ± standard deviation. ****p < 0.0001, unpaired t-test