Fig. 1

Comparative Analysis of Microbiota Composition and Metabolic Profiles in C57Ola_Ntg and 129Sv_Ntg Mice at 12 weeks’ age. (A) Boxplots showcasing alpha diversity indices (Observed Richness, Shannon index, Evenness) in fecal samples. Statistical differences were evaluated using a paired Wilcoxon signed-rank test. P-values less than 0.05 were considered statistically significant. (B) Principal coordinates analysis (PCoA) according to the Hellinger distance computed on genera abundances. Results of the permutational multivariate analysis of variance (PERMANOVA) are also shown based on the first two coordinates. (C) Circular heatmap representing the differentially abundant taxa in C57Ola versus 129 Sv mice samples: concentric circles represent taxonomic ranks from phylum (P) to genus (G); yellow shades indicate positive logFC values, whereas blue shades indicate negative logFC values correlations; the intensity of colors is proportional to logFC values. (D) Box plot reporting the statistically significant different free fatty acids among the two Ntg strains. (E) Computed Linear Discriminant Analysis (LDA) scores representing significantly differential KEGG Pathway Expression (LDA > 2.5) between 129Sv_Ntg and C57Ola-Ntg mice