Fig. 4

AQP4 canonical isoform expression in AQP4ex-KO mice post-AWI. (a) Typical immunoblot of M1 and M23 isoforms detection in three untreated and treated mice; Western blot experiments are representative and the samples shown belong to the same electrophoretic run. (b) Results of the densitometric aanalysis shown as the percentage variation compared to baseline (red dashed line) of AQP4. Note that after 20 min of AWI canonical isoforms significantly increased. (Red asterisk indicates the Student’s t-test significant differences for the comparison with basal level, while black one the comparison between different time points, *p < 0.05, **p < 0.01, n = 10)