Fig. 3

AQP4 isoforms protein expression in WT mice after different time points of AWI. (a, c, e) Immunoblot results of AQP4, AQP4ex and p-AQP4ex expression in three different untreated (CTRLs) and treated mice at 10, 20 and 30 minuses post-AWI. Western blot experiments are representative and the samples shown belong to the same electrophoretic run. (b, d, f) Results of the densitometric analysis shown as the percentage variation compared to baseline (red dashed line) of AQP4, AQP4ex and P-AQP4ex respectively (Red asterisk indicates the Student’s t-test significant differences for the comparison with basal level, while the black one indicates the comparison between different time points). (g) Ratio between p-AQP4ex and AQP4ex showing that only half of additional AQP4ex was phosphorylated at 20 min. Data are expressed as means ± SEM (*p < 0.05; One-Way ANOVA n = 10)