Table 1 Overview of antibodies, plasmids, and products used
Reagent or resource | Source | Identifier |
|---|---|---|
Antibodies | ||
α-tubulin (ICC 1:50/WB 1:500) | Cell Signaling Technology, Danvers, US | 2125S |
GAPDH (1:5000) | Thermo Fisher Scientific, Waltham, US | AM4300 |
Tom20 (ICC 1:50/WB 1:500) | Santa Cruz Biotechnology, Dallas, US | Sc-17764 |
VCP (1:1000) | Thermo Fisher Scientific, Waltham, US | MA3-004 |
Alexa Fluor 488 donkey anti-rabbit (1:1000) | Invitrogen, Waltham, US | A21206 |
Alexa Fluor 555 donkey anti-mouse (1:1000) | Invitrogen, Waltham, US | A31570 |
Mitochondrial respiratory activity chemicals | ||
Hepes | Sigma-Aldrich, St. Louis, US | H3375 CAS: 7365-45-9 |
Sucrose | Thermo Fisher Scientific, Waltham, US | S-8600-60 CAS: 57-50-1 |
KH2PO4 | Sigma-Aldrich, St. Louis, US | P0662 CAS: 7778-77-0 |
KCl | Merck, Darmstadt, Germany | 104936 CAS: 7447-40-7 |
EGTA | Sigma-Aldrich, St. Louis, US | E4378 CAS: 67-42-5 |
MgCl2 | Sigma-Aldrich, St. Louis, US | M2670 CAS: 7791-18-6 |
BSA (fatty acid free) | Sigma-Aldrich, St. Louis, US | A6003 CAS: 9048-46-8 |
NaOH | Merck, Darmstadt, Germany | 109913 CAS: 1310-73-2 |
Compounds | ||
α-Tocopherol (75 µM; 1 h) | Sigma-Aldrich, St. Louis, US | T3251 CAS: 10191-41-0 |
Bongkrekic acid (10 µM; 1 h) | Sigma-Aldrich, St. Louis, US | B6179 CAS 11076-19-0 |
Carbachol (15 µM, 10.5 min) | Sigma-Aldrich, St. Louis, US | C4382 CAS: 51-83-2 |
Cyclosporin A (1 µM and 10 µM; 1 h) | Sigma-Aldrich, St. Louis, US | 30024 CAS: 59865-13-3 |
FK506 (10 nM and 25 µM; 1 h) | Abcam, Cambridge, UK | Ab120223 CAS: 104987-11-3 |
MCUi4 (TMRM and mPTP assay:1 and 3 µM; 1 h/ calcium measurements 2.5 or 5 µM; 1.5 h) | Tocris, Bristol, UK | 7195 CAS: 371924-24-2 |
N-acetyl-L-cysteine (NAC) (1Â mM; 2Â h) | Sigma-Aldrich, St. Louis, US | A9165 CAS: 616-91-1 |
NIM811 (3 µM; 1 h) | MedChemExpress, New Jersey, USA | HY-P0025 CAS: 143205-42-9 |
Thapsigargin (2.5 µM, 10.5 min) | Enzo Biochem, NY, USA | BML-PE180 CAS: 67526-95-8 |