Fig. 4

Metabolic characterization of the established models. A PCA plot of the three cell cell lines and two media conditions (Blue = GSC923, Red = 403L, Green = 403H, Circles = Media with 1 mM glutamine, Triangles = Media without glutamine). B Bar graph showing a total concentration of tested metabolites normalized to cell count in each cell line. C Statistically significant metabolites for the 403H vs 403L comparison plotted on the major metabolite pathways. D Energy map showing real-time measurement of maximal OCR and ECAR levels in live 403L and 403H cells. E Hyperpolarized ¹³C pyruvate NMR spectroscopy measuring Lac/Pyr ratio in 403L and 403H. F Western blot of LDHA and LDHB expression in 403L and 403H. G LDH activity in both cell lines