Fig. 8

Analysis of upregulated genes in Y-33075 exposed retinal explants. a Heatmap depicting the treatment response to either the control solvent or Y-33075, along with the expression variability for each sample among the 13 significantly upregulated genes in the Y-33075 group compared to the control solvent group. b Read count plots for the neurofilament gene markers Nefh and Prph, the neurotransmitter gene marker Nrn1, and the RGC-specific gene markers Rbpms and Slc17a6 in retinal explants at DEV 0 and DEV 4, which are among the top 13 most highly expressed genes. c Read count plots of the RGC-specific gene marker Pou4f1, the neuronal gene Thy1 in retinal explants at DEV 0 and DEV 4. Unpaired t tests or Mann–Whitney tests were performed between the control solvent and 50 µM Y-33075-treated groups at DEV 4. Error bars indicate the standard error of the mean. *p < 0.05, ***p < 0.001, ****p < 0.0001)