Fig. 6

Analysis of downregulated genes in Y-33075-exposed retinal explants. a Heatmap showing the response to treatment corresponding to the control solvent or Y-33075 and the variability of expression for each sample for the 25 most significantly downregulated genes in the Y-33075 group versus the control solvent group. b RNA-seq-based read count plot gene expression analysis of the TOP 25 downregulated genes in retinal explants at DEV 0 and DEV 4. c RNA-seq-based read count plot gene expression analysis of microglial and glial cell markers in retinal explants at DEV 0 and DEV 4. d RNA-seq based read count plot gene expression analysis of M1 inflammatory markers in retinal explants at DEV 0 and DEV 4. e Read count plots of the inflammasome component gene markers Nlrp1, Nlrp3, Pycard, and Casp1 and their downstream targets Il1b and Il18 in retinal explants at DEV 0 and DEV 4. f Read count plots of the apoptosis gene markers Casp3 and Casp8 and the ferroptosis gene marker Ptgs2 in retinal explants at DEV 0 and DEV 4. Unpaired t tests or Mann–Whitney tests were performed between the control solvent and 50 µM Y-33075-treated groups at DEV 4. Error bars indicate the standard error of the mean. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)