Fig. 1

Trem2-deficiency exacerbates age-related myelin damage in the striatum. (A) Representative fluorescent images of striatal white matter from 6-, 12-, and 18-month-old B6 and Trem2^−/− mice stained for MBP (gray) with binary insets highlighting the fragmentation. (B) Quantification of MBP percent area and (C) fragmentation in 6-, 12-, and 18-month striatum. (D) Representative western blot analysis and (E) densiometric quantification of white matter proteins CNPase, dMBP, and MBP in white matter homogenates from 6-, 12-, and 18-month-old B6 and Trem2^−/− mice. Immunoblot quantification was normalized to 6-month B6 samples. All experiments used n = 4 (equal males and females) per group. Two-way ANOVA with Tukey’s post-hoc test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) was used for statistical analysis. Error bars represent SEM