Fig. 4
Dnmt1 genetic loss during SHH-MB development. a Representative H&E stainings and immunohistochemistry for DNMT1-C, Ki67, and cleaved Caspase 3 (CC3) in tumors from Math1-cre::SmoM2Fl/+, Math1-cre::Dnmt1Fl/+::SmoM2Fl/+ and Math1-cre::Dnmt1Fl/Fl::SmoM2Fl/+ mice at P5. b Quantification of Ki67- (left) and CC3-positive cells (right) in tumors from mice with indicated genotype at P5 (n = 4, Fisher´s exact test). c Kaplan–Meier curves of Math1-cre::SmoM2Fl/+ mice (n = 11) compared to heterozygously (n = 11) and homozygously (n = 11) Dnmt1-depleted Math1-cre::SmoM2Fl/+ mice. Significance in survival was determined using the log rank (Mantel-Cox) test. 4 × magnification, scale bar, 500µm; 20 × magnification, scale bar, 50µm. All graphs display mean ± SD. * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001