Fig. 4

Presence of disease-related proteins in wasteosomes. a1-a3 Wasteosomes from the hippocampus of a PSP patient stained with anti-p62 (green) and anti-tau (red, a1), and not labelled with anti-pTDP43 (red, a2) and anti-FUS (red, a3). The white arrows in a1 show tau staining in the periphery of wasteosomes. b1-b3 Wasteosomes from the hippocampus of a CBD patient stained with anti-p62 (green) and anti-tau (red, b1), and not labelled with anti-pTDP43 (red, b2) nor anti-FUS (red, b3). The white arrows in b1 show tau staining in the periphery of wasteosomes. c1-c3 Wasteosomes from the hippocampus of an FTLD-TDP patient stained with anti-p62 (green) and anti-pTDP43 (red, c2), and not labelled with anti-tau (red, c1) nor anti-FUS (red, c3). The white arrow in c2 shows pTDP-43 staining in the periphery of a wasteosome. d1-d3 Wasteosomes from the hippocampus of an FTLD-FUS patient stained with anti-p62 (green) and anti-FUS (red, d3), and not labelled with anti-tau (red, d1) nor anti-pTDP43 (red, d2). The empty arrow points out a FUS inclusion in the central area of a wasteosome (red, d3). Nuclei are stained with Hoechst (blue). CBD: corticobasal degeneration; FTLD: frontotemporal lobar degeneration; PSP: progressive supranuclear palsy. Scale bar: 10 µm