Fig. 4
From: Cryo-EM structures reveal tau filaments from Down syndrome adopt Alzheimer’s disease fold
GO-AT8 antibody affinity-grid assembly and isolation of tau filaments for cryo-EM. A Schematic showing the affinity-grid assembly procedure for the attachment of AT8 anti-tau antibodies to the GO grid surface. B Control cryo-EM micrograph image for which protein G was not included in the affinity-grid assembly prior to incubation with tau filaments and vitrification. C Representative cryo-EM micrograph images following GO-AT8 affinity-grid isolation of tau filaments from DS case 2 (left) and DS case 4 (right). Fewer filaments are observed in case 4 due to the low overall abundance of tau deposits