Fig. 5

Astrocytic αSynΔC Positive Pathology in TgM20^+/- Mice Seeded with MSA Lysate and PFFs. Representative co-immunofluorescence images from TgM20^+/- mice brain sections seeded with (a) MSA or (b) PFFs. Sections were double-labeled with GFAP, an astrocyte marker, and αSynΔC-specific antibodies 2G5 (αSynΔC103), 1A2 (αSynΔC114), 10A4 (αSynΔC122), 5C1 (αSynΔC125), 2G7 (αSynΔC129). Scale bars: 50 µm; 25 µm (insets). c Images were assessed based on the frequency of αSynΔC variants co-localizing with GFAP+ cells, categorized as frequent, sparse, rare, or absent (none). The images shown highlights αSynΔC variants that were observed either frequently or sparsely within GFAP+ cells. Representative images depicted are of the subiculum (2G5 in a and 2G5 in b), the hippocampal formation (5C1 in a), and the entorhinal cortex (10A4 in b and 5C1 in b)