Fig. 4

Astrocytic αSynΔC inclusion pathology in TgM83^+/- mice seeded with MSA lysates and PFFs. Representative co-immunofluorescence images from TgM83^+/- mice brain sections seeded with (a) MSA lysates or (b) PFFs. Sections were double-labeled with GFAP, an astrocyte marker, and αSynΔC-specific antibodies 2G5 (αSynΔC103), 1A2 (αSynΔC114), 10A4 (αSynΔC122), 5C1 (αSynΔC125) or 2G7 (αSynΔC129). Scale bars: 50 µm; 25 µm (insets). c Images were assessed based on the frequency of αSynΔC variants co-localizing with GFAP+ cells, categorized as frequent, sparse, rare, or absent (none). The images shown highlight αSynΔC variants that were observed sparsely within GFAP+ cells. Representative images depicted are of the medulla (2G5 in a), the periaqueductal grey areas (5C1 in a), and the hippocampal formation (5C1 in b)