Fig. 3
Comparison of strain specific regional deposition of αSynΔC in the CNS of TgM20+/- mice. a, b Representative images of αSyn and αSynΔC immunohistochemical staining in TgM20+/- mice seeded injected with (a) MSA lysates or (b) PFFs. Immunohistochemistry was performed with antibodies 3H19 (αSyn 110–119) 2G5 (αSynΔC103), 1A2 (αSynΔC114), 10A4 (αSynΔC122), 5C1 (αSynΔC125) or 2G7 (αSynΔC129). Selected brain regions, including HPF (hippocampal formation), SUB (subiculum), MY (medulla), and SP (spine) are depicted. Scale bar: 100 µm. c, d Semi-quantification comparing the regional distribution and burden of 3H19 and αSynΔC positive inclusions. Additional abbreviations: CTX (cortex), PIR (piriform cortex), AMY (amygdala), TH (thalamus), HY (hypothalamus), ENTI (entorhinal cortex), PAG (periaqueductal gray), and MB (midbrain). e–i Heatmap distributions of αSynΔC pathology