Fig. 8

Western blot analysis of Akt, Erk and GSK3β signaling in the healthy and glaucoma retinas in response to AAV-Tau modulation. A Immunoblots of pAkt^(Ser473) (1:2000), Akt(1:1000), pErk^{(Thr202/Tyr204)} (1:2000), Erk(1:1000), pGSK3β^(Ser9)(1:1000), and GSK3β(1:1000) in Tau overexpressing retinas in normal and glaucoma conditions compared to the respective control retinas and β-actin (1:5000), used as a loading control. B Quantification of pAkt^(Ser473) band intensities measures as relative fold changes to the total Akt expression. C, D Quantitative analysis of pErk1^{(Thr202/Tyr204)} and pErk2^{(Thr202/Tyr 204)} levels compared to the total Erk1 and Erk2. E Quantitative analysis of pGSK3β^(Ser9) band intensities and their relative fold changes compared to the GSK3β expression (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n = 3 each group, One-way ANOVA analysis with Tukey’s multiple comparison test). F Immunoblots of pAkt^(Ser473), Akt, pErk^{(Thr202/Tyr204)}, Erk, pGSK3β^(Ser9), and GSK3β in AAV-mTau knockdown retinas in normal and glaucoma conditions compared to the respective control retinas and β-actin used as a loading control. G Quantitative analysis of pAkt^(Ser473) band intensities and its relative fold change to total Akt expression. H, I Quantitative analysis of pErk1^{(Thr202/Tyr204)} and pErk2^{(Thr202/Tyr 204)} compared to the total Erk1 and Erk2 levels. J Quantitative analysis of pGSK3β^(Ser9) band intensities to the total GSK3β expression (***P < 0.001, ****P < 0.0001, n = 3 each group, One-way ANOVA analysis with Tukey’s multiple comparison test)