Fig. 5

Western blotting (WB) analysis of tubulin and its post-translational modifications in AAV-mediated Tau modulated retinas. A WB of retinal lysates of the control and glaucomatous C57BL/6 mice eyes with Ac-tubulin (1:1000), Tyr-tubulin (1:1000), and total tubulin (1:1000) antibodies. B Quantification of relative intensities of the WB bands for Ac-tubulin and C Tyr-tubulin in retaliation to the total tubulin levels (n = 3, t-test, **P < 0.01, ***P < 0.001). D WB of retinal lysates of mice eyes transduced with AAV-GFP control and AAV-mTau construct (Tau overexpression) probed with Ac-tubulin, Tyr-tubulin, and total tubulin and their quantified E relative band intensities for Ac-tubulin and F Tyr-tubulin (**P < 0.01, ***P < 0.001, t-test, n = 3 per group, One-way ANOVA analysis with Tukey’s multiple comparison test,). G WB of retinal lysates from AAV-scrambled shRNA and AAV-mTau KD transduced mice probed with Ac-tubulin, Tyr-tubulin, and total tubulin antibodies. H Quantification of relative WB band intensities of Ac-tubulin and I Tyr-tubulin plotted to the total tubulin (n = 3) (***P < 0.001, ****P < 0.0001, t-test, n = 3 per group, One-way ANOVA analysis with Tukey’s multiple comparison test,)