Fig. 8

Hyperoside inhibits DNA-stimulated pro-inflammatory responses in BV-2 cells. (a) ELISA was performed to quantify ctDNA-stimulated production of TNF, IL6, and INFB 3 h after the indicated treatments. (b) Real-time qPCR analyses of the expression of Ccl5, Cxcl10, Ifnb, Il6 and Tnf in BV-2 cells collected 3 h after the indicated treatments. Relative fold change was normalized against the vehicle-treated BV-2 cells. (c) Western blotting analyses of the level of p-TBK1 and TBK1 in BV-2 cells collected 3 h after the indicated treatments. β-actin was probed as the internal control. Relative fold change was normalized against vehicle-treated BV-2 cells. (d) ELISA was performed to quantify ctDNA-stimulated production of TNF 3 h after the indicated treatments. Data were expressed as mean ± SEM (n = 6 per group). ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ns, not significant