Fig. 5

Post-light damage treatment of hyperoside lowers the retinal expression of genes involved in cytosolic DNA-sensing pathway. (a) Gene set enrichment analysis was derived from our previously published RNA-sequencing data [10]. (b) Heatmap visualization of the representative genes identified in cytosolic DNA-sensing pathway [10]. (c) Hyperoside was administered to the light-exposed mice 3 h after illumination and the retinas were isolated 24 h later. The retinal expression of the indicated genes was analyzed by real-time qPCR. Relative fold change was normalized against NLE (y-axis, log10 scale). Data were expressed as mean ± SEM (n = 6 per group). ^**Compared to NLE, P < 0.01; ^***compared to NLE, P < 0.001; ^#compared to LE, P < 0.05; ^##compared to LE, P < 0.01; ^###compared to LE, P < 0.001; ns, not significant. NLE, the vehicle-treated mice without the experimental light exposure; LE, the vehicle-treated light-exposed mice; HYP, the hyperoside-treated light-exposed mice; ES, enrichment score; NES, normalized enrichment score; FDR, false discovery rate