Fig. 10

Hyperoside directly interacts with and inhibits cGAS. (a) MD simulations of hyperoside-mouse cGAS complex. (b) Binding mode of hyperoside to cGAS. (c) SPR analyses of the hyperoside-human cGAS interaction. (d) CETSA assessment of the binding of hyperoside and cGAS protein in BV-2 cells. β-actin was the internal reference. The data were representative of three independently repeated experiments. (e) Measurement of cGAS activity in a cell-free assay by competitive ELISA. cGAS inhibitor CU-76 was included as the positive control. Data were expressed as mean ± SEM (n = 6 per group). ^*P < 0.05; ^**P < 0.01. RMSD, root mean square deviation; ns, nanosecond