Fig. 6

NPFF-immunopositive cells/mm³ in the allocortex of n = 9 control versus n = 22 patients with neurodegenerative disorders. a/c Cell counts in the gray/white matter of the entorhinal region (ER-GM, ER/WM) and b/d hippocampal formation (HF-GM, HF-WM). Males (black) and females (blue). For the demographics and neuropathological diagnoses of the controls (n = 6 ER, n = 5 HF), sporadic ALS patients (n = 3 ER, n = 8 HF), AD patients (n = 3 ER, n = 5 HF), PiD patients (n = 0 ER, n = 2 HF), and schizophrenia (n = 1 ER, n = 1 HF), see Supplementary Table 1. Quantification of NPFF-positive cells was normalized to the volume of analyzed surface area. Data are presented as mean ± SEM and analyzed by one-way ANOVA followed by Dunnett’s multiple comparisons test. Statistically significant differences are indicated (*p < 0.05 **p < 0.01). The hippocampal stratum oriens displayed only a few axon-like NPFF-positive cellular processes, whereas in the pyramidal cell layer of the subiculum and CA1-CA3 sectors a web-like NPFF-positive plexus existed. The stratum glomerulosum-moleculare and perforant path were marked by remarkably dense accumulations of axon-like NPFF-positive cellular processes