Fig. 4

NPFF-somatostatin and NPFF-nNOS double immunostaining in the frontal gyrus. a–d NPFF (brown chromogen DAB) and somatostatin (blue chromogen, SK4700 Vector) double immunostaining. Overview in a showing three cells, each of which is shown at higher magnification in b–d NPFF/somatostatin-positive cell within solid framed area in a is shown at higher magnification in b. In c the cell is NPFF-positive (DAB) but negative for somatostatin (dashed framed area in a); in micrograph d, the cell shown (arrow in a) is immunopositive only for somatostatin (SK4700 Vector). Case 9: female, 87 years of age. e–h NPFF (brown chromogen DAB) and neuronal NOS (blue chromogen, SK4700 Vector) double immunostaining. Overview in e contains two nNOS immunopositive cells (frames) shown at higher magnification in f (NPFF/nNOS-positive) and h (immunopositive only for nNOS, SK4700 Vector). The cell in g from elsewhere in the same slide as e is NPFF-positive (DAB) but nNOS-negative. Case 8: male, 83 years of age. 100 µm PEG-embedded sections from frontal gyrus (a-g). Scale bar in a is valid for e; scale bar in b also applies to c, d, and f–h