Fig. 4

Correlation between paired primary and recurrent GBMs at the transcriptomic and genomic levels. A Transcriptomic similarity (measured by the TROM scores) between two GBM samples selected from the GLASS dataset (87 pGBM and 87 rGBM samples). The similarity between a sample and itself was not shown. The red box represented the 87 P-R paired GBM samples. P value was assessed using permutation test (see the text). B Correlation between the PC1s of genes expressed in paired primary and recurrent GBMs for the GLASS cohort. C Correlation between the TMB values (mutations/Mb) in paired primary and recurrent GBMs for the GLASS cohort. For (B) and (C), the orange, blue, and green solid dots represented the cases with TTR ≤ 6 months (23 pairs), TTR of 7–12 months (33 pairs), and TTR < 12 months (31 pairs), respectively. Numbers in parentheses indicated the fitting levels (measured by the RMSE) of the cases in the corresponding TTR groups; P values were assessed using permutation test (see the text). Correlation coefficients and the corresponding P values were evaluated using the Pearson’s correlation analysis. D Percentages of tumor hypermutation for the GBM patients with short (< 8.66 months; n = 52), medium (8.66–16.7 months; n = 52), and long (> 16.7 months; n = 51) PFIs in the G-SAM dataset. P values were assessed using one-tailed Fisher’s exact test. pGBM, primary GBM. rGBM, recurrent GBM. *P ≤ 0.05, **P ≤ 0.01