Fig. 5

Human brain αsyn pathology is phosphatase resistant. Whole section images of transverse midbrain sections from A PD and B MSA cases stained for PSER129 with (+CIAP) or without CIAP pretreatment. High magnification images of substantia nigra of C PD and MSA. D Representative thresholding of images prior to quantification. E Quantification of staining in PD and MSA brain with or without CIAP. F Proteins extracted from tissues that either did or did not have αsyn aggregates. Proteins were separated by SDS-PAGE and blotted onto PVDF membranes which were incubated with and without CIAP. Blots were probed for both PSER129 and αsyn. All sections developed with nickel-DAB Chromogen (black/purple) and counterstained with methylgreen n = 5–10. Scale bars for A and B = 2 mm, for C = 25 µm, for D = 150 µm. ns = non-significant