Fig. 4

CIAP-resistant PSER129 partially colocalizes with PK-resistant αsyn. Brain sections from M83 mice unilaterally injected with αsyn PFFs into the OB GCL were multiplex labeled for CIAP resistant PSER129 (CIAP-PSER129, red) and PK resistant αsyn (PK-αsyn, green) and nuclei (DAPI, blue). Representative images show labeling in the brainstem (BS) (A), OB and PA (B). Arrows denote the position of cell processes labeled for CIAP-PSER129 but lack overlapping PK-αsyn. C Single plane image of a dysmorphic neurite. D Pearson colocalization coefficients for PK-αsyn and CIAP-PSER129 in the OB and PA. E Plot of pixel intensity values for confocal image of CIAP-PSER129 and PK-αsyn in PA. Pearson correlation coefficient (R) shown on plot. n = 3–4. Scale bars for A = 25 µm, B, C = 20 µm