Fig. 3

Seeded aggregates in M83 mice are CIAP resistant. M83 mice were unilaterally injected with αsyn PFFs into the OB GCL. 6 months following injections mice were perfusion fixed. A PSER129 staining in the brain of M83 mouse injected with PFFs without or without CIAP treatment. Red star denotes the OB injected with PFFs. Red line annotates the PA of the PFF injected hemisphere. B High magnification images of CIAP treatment tissue sections from M83 mice injected with either PBS or PFFs. C High magnification images of PA from mouse brain sections treated with CIAP for either 16 h or 70 h. D Representative thresholding of images prior to quantification. E Quantification of PSER129 signal in the OB. ****Fisher’s LSD, p < 0.0001, n = 3–4. Scale bar for B, C, and D = 50 µm