Fig. 2

Seeded aggregates in WT mice are CIAP resistant. Mice were bilaterally injected with either PBS or PFFs into the OB GCL. Two months following injection, animals were euthanized with a mixture of ketamine/xylazine followed by transcardial perfusion fixation. Brain sections across the neuroaxis were stained for PSER129 with and without CIAP pretreatment. A Whole section images of PSER129 stained brain images across the neuroaxis, with (+CIAP) or without CIAP (−CIAP) pretreatment. B Low and C high magnification images of select brain regions. D Representative images of signal thresholding and E subsequent quantification of PSER129 immunoreactivity in the OB GCL.***Fisher's LSD, p<0.001. **Fisher's LSD, p<0.01. OB olfactory bulb, AMYG amygdala, NaAc nucleus accumbens, HIP Hippocampus, GP Globus pallidus, HYPO hypothalamus, SNPr substantia nigra pars reticulata, MCL mitral cell layer, GCL granule cell layer, EC entorhinal cortex. All sections developed with nickel-DAB Chromogen (black/purple) and counterstained with methylgreen. n = 3. Scale BAR for B = 100 µm, for C = 25 µm, D = 50 µm