Fig. 5

Hyaluronan levels in slice-CM of HPMB-OSCs. (a) Hyaluronan levels determined by ELISA in slice-CM of HPMB-OSCs obtained from PSY3, the MLD patient, and the AxD patient (from top to bottom) cultured without (pink) or with (blue) addition of hCSF. The dotted line represents the–hCSF and + hCSF medium controls, set at 100%. Hyaluronan levels are presented as percentage of the corresponding medium control. Data are displayed as mean of technical replicates and hence no statistical analysis could be performed. (b) GFAP staining of slices cultured without (left) or with (right) hCSF obtained from PSY3, the MLD patient and AxD patient (from top to bottom), showing decreased immunoreactivity for GFAP in slices supplemented with hCSF