Fig. 4

Comparative proteomic analysis. (A) Schematic depicting the process of analysing myofibers obtained from controls and CFZS patients (5 fibers collected per sample tube). (B) Venn diagram depicting detected proteins that were significantly upregulated and downregulated in CFZS patients as well as those that remained unchanged between experimental groups. (C) Volcano plot displaying Log2 fold change (Log2 fc) against Log10 p-value. Dark blue dots indicate p < 0.05 controls (aka. downregulated in CFZS) whereas red dots indicate p < 0.05 for CFZS patients (aka. upregulated in CFZS). Non-significant (p > 0.05) proteins are highlighted in grey. The top 10 most significant proteins for each experimental group have been annotated in blue (downregulated in CFZS) or red (upregulated in CFZS). (D) A heat map was created to illustrate the proteins with the greatest fold change, all proteins included possessed a Log2 FC > 1.5. For readability gene names rather than protein names were included in the heap map