Fig. 2

Intravitreal MPTP administration drives retinal ganglion cell loss. A Retinal ganglion cells were fluorescently labelled in whole-mounted retina with anti-RBPMS (magenta). B There were no significant differences between the naïve and vehicle control (green) groups. RBPMS density significantly decreased relative to the vehicle control at 7, 14 and 21 days post-injection for 5 mg/mL (orange) and 50 mg/mL (red) MPTP. C The thickness of all retinal layers (GCL, IPL, INL, OPL, and ONL) was measured from cross-sections. For all retinal layers, there were no significant differences between the vehicle control (green) and 5 mg/mL MPTP (orange) or 5 mg/mL MPTP (red) at 7 days post MPTP injection. D All amacrine cells were fluorescently labelled in retinal cross-sections with anti-Prox1 antibodies. Images captured with a 5 × objective were analyzed for Prox1 signal intensity, images captured with a 20 × objective were analyzed for Prox1+ cell soma counts. This data was plotted. There were no significant differences in signal intensity between vehicle control (green) and 5 mg/mL MPTP (orange) or 50 mg/mL MPTP (red). The number of Prox1+ cells, the total number of INL cells (relative to the measured INL area), and the relative density of Prox1+ cells (Prox1+ cells/total cell number) did not significantly change over the different conditions. There were no significant differences in between vehicle control (green) and 5 mg/mL MPTP (orange) or 50 mg/mL MPTP (red). Scale bar = 20 µm in A and D (20 ×), 200 µm in D (5 ×)