Fig. 1

Intravitreal MPTP administration does not result in TH⁺ cell loss. A Dopaminergic amacrine cells were labelled with anti-TH antibodies in whole-mounted retinas. B The density of TH+ cells was not significantly different between the naïve and vehicle control (green) groups and between the vehicle control and the groups injected with 5 mg/mL MPTP (orange) or 50 mg/mL MPTP (red) at 7, 14, or 21 days post-injection. C The dendritic fields of the TH+ amacrine cells were reconstructed using Imaris. D From the reconstructions, the total dendrite length, total dendrite volume, number of varicosities, and total varicosity volume were quantified. There are no significant differences between the naïve and vehicle control (green) groups, the groups injected with 5 mg/mL MPTP (orange), or 50 mg/mL MPTP (red) at 7, 14 and 21 days post-injection for all three of these parameters supporting a lack of dendritic changes. Scale bar = 100 µm in A and C