Fig. 4

Integrin α6 knockdown improves DIPG drug response to panobinostat. a Schematic of experimental design to assess the role of integrin knockdown (KD) on panobinostat response within DIPG-neural assembloids. b Representative immunostaining for Cleaved Caspase 3 (CC3, red) and DAPI (blue) at the fusion interface within the DIPG-neural assembloids when treated with 0 or 200 nM panobinostat. Scale bar, 100 µm. c Quantification of the relative degree of apoptosis based on CC3 immunostaining at the fusion interface normalized by DAPI and relative to the untreated assembloid control group in each respective KD group (n = 3 assembloids per group). *p < 0.05 by one-way ANOVA with Tukey’s multiple comparisons test. Data reported in c represent mean value ± SD