Fig. 3

Laminin-associated integrin knockdown improves DIPG response to radiation in a 3D DIPG-neural assembloid model. a Schematic of experimental design to assess the role of integrin knockdown (KD) on radiation response within DIPG-neural assembloids. b Representative immunostaining for Cleaved Caspase 3 (CC3, red) and DAPI (blue) at the fusion interface within the DIPG-neural assembloids when irradiated at 0, 5, and 20 Gy. Scale bar, 100 µm. c Quantification of the relative degree of apoptosis based on CC3 immunostaining at the fusion interface normalized by DAPI and relative to the untreated assembloid control group in each respective KD group (n = 3 assembloids per group). d Representative immunostaining for γH2AX (red) and DAPI (blue) at the fusion interface within the DIPG-neural assembloids when irradiated at 0, 5, and 20 Gy. Scale bar, 100 µm. e Quantification of the relative degree of DNA damage based on γH2AX immunostaining at the fusion interface normalized by DAPI and relative to the untreated assembloid control group in each respective KD group (n = 3 assembloids per group). *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA with Tukey’s multiple comparisons test. Data reported in c and e represent mean value ± SD